Linda Brubaker ¹, Jean-Philippe F Gourdine ², Nazema Y Siddiqui ³, Amanda Holland ⁴, Thomas Halverson ⁵, Roberto Limeria ⁶, David Pride ^7 8, Lenore Ackerman ⁹, Catherine S Forster ¹⁰, Kristin M Jacobs ¹¹, Krystal J Thomas-White ¹², Catherine Putonti ^5 13, Qunfeng Dong ^14 15, Michael Weinstein ^16 17, Emily S Lukacz ¹, Lisa Karstens ¹⁸, Alan J Wolfe ^5 6

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Abstract

Urobiome research has the potential to advance the understanding of a wide range of diseases, including lower urinary tract symptoms and kidney disease. Many scientific areas have benefited from early research method consensus to facilitate the greater, common good. This consensus document, developed by a group of expert investigators currently engaged in urobiome research (UROBIOME 2020 conference participants), aims to promote standardization and advances in this field by the adoption of common core research practices. We propose a standardized nomenclature as well as considerations for specimen collection, preservation, storage, and processing. Best practices for urobiome study design include our proposal for standard metadata elements as part of core metadata collection. Although it is impractical to follow fixed analytical procedures when analyzing urobiome data, we propose guidelines to document and report data originating from urobiome studies. We offer this first consensus document with every expectation of subsequent revision as our field progresses.